Kinetics of Triterpenoid Production and Nutrient Consumption in Birch (Betula platyphylla) Suspension Cell p
Effects of the basic media and inoculum size on birch cell growth and the triterpenoid production were investigated using birch suspension cells, on this basis, the kinetic relationships of birch cell growth, triterpenoid production and nutrient consumption were also analyzed. The results showed the opbimal conditions for the birch cell growth and triterpenoid production were 20 ～ 30 g· L-1(FW) of inoculum size, in B5 medium. The kinetics analysis for birch cell suspension culture showed that triterpenoid production was increasing with the birch cell growth during a cell subculture cycle. During the teriod, the maximum specific growth rate was 0. 18 on the 12th day, and the maximum triterpenoid synthesis rate was 0.73 mg· g-1 on the 9th day. Sugar, NO3- , NH+4 and PO34- in the culture medium were nearly used up on the 9th day, The content of sugar, NO-3 , and PO34- in birch cell were highest on 6th day, but the content of NH+4 in birch cell were higher on 3th day. The results snggested that the nutrient deficiencies in medium wonld limited the cell rapid multiplication and triterpenoid content accumulation.
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