首页|3个属竹叶中黄酮类化合物的比较

3个属竹叶中黄酮类化合物的比较

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采用高效薄层色谱法(HPTLC)检测箣竹属、牡竹属及刚竹属11个竹种竹叶中牡荆苷、异牡荆苷、荭草苷、异荭草苷和苜蓿素.采用自动多级展开法,5种黄酮类化合物分离效果良好,其RF值分别为0.25,0.32,0.38,0.47和0.88,回收率在79.01％ ～ 106.85％之间.3属11种竹叶中黄酮类化合物种类和含量具有差异,紫竹中5种黄酮含量总和最高,为0.132％;麻竹中5种黄酮含量总和最低,为0.015％.
Comparison of Flavonoids in the Leaves of Three Genera of Bamboo
Isoorientin,orientin,isovitexin,vitexin and tricin in the leaves of three genera 11 bamboos been compared by a simple high-performance thin-layer chromatographic (HPTLC) method.The multistage development was performed in Automated Multiple Development (AMD2) and solvents with different ratios were used as mobile phase.The developed plates were scanned by TLC Scanner3.The components were separated well and RF of the five flavonoids 0.25,0.32,0.38,0.47 and 0.88 respectively.The method was validated for precision,accuracy,selectivity and repeatability,and expressed as coefficient of variation (CV [％]).The kinds of flavonoids and the concentrations in the eleven species bamboo of three different genera were significantly different.Phyllostachys nigra the maximum total flavonoids of 0.132％,and the minimum of 0.015％.The results would be useful in extracting natural bamboo-leaf flavonoids.

HPTLCbamboo-leaf flavonoidsqualitative and quantitative analysisBambusaDendrocalamusPhyllostachys

魏琦、岳永德、汤锋、孙嘏

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国际竹藤中心 北京100102

高效薄层色谱(HPTLC) 竹叶黄酮 定性及定量分析 箣竹属 牡竹属 刚竹属

This research was supported by the State Forestry Administration,P.R.China 948 ProjectNational Science and Technology Infrastructure ProgramBasic Science Research Fund Program of the International Centre for Bamboo and Rattan (ICBR)

2009-4-612012BAD23B0312618-6

2013

10.11707/j.1001-7488.20131020

林业科学
中国林学会

林业科学

CSTPCDCSCD北大核心
影响因子：1.272
ISSN：1001-7488
年,卷(期)：2013.49(10)
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