Somatic Embryogenesis of Picea asperata Induced from Immature Embryos
Immature zygotic embryos of Picea asperata were used as explants to investigate effects of cone collection dates,genotypes,different concentration of auxin and cytokinin on the induction of embryogenic callus. At the same time, the key factors affecting the differentiation and maturation of somatic embryos were also comprehensively investigated. The results showed that the optimal medium for induction and proliferation of embryogenic callus was 1/2 LM + 2. 2 mg·L -1 2,4-D + 1. 1 mg·L -16-BA + 10 g·L -1 sucrose + 2 g·L -1 gellan gum + 1g·L -1 casein hydrolysate. The best medium for differentiation of somatic embryos was 1/2 LM + 24 mg·L -1 ABA + 50 g·L -1 PEG4000 + 30 g·L -1 sucrose + 1 g· L -1 activated charcoal+ 4 g·L -1 gellan gum + 1 g·L -1 casein hydrolysate. The suitable medium of germination was 1/4 LM + 20 g·L -1 sucrose + 1 g·L -1 AC + 4 g·L -1 gellan gum + 1 g·L -1 casein hydrolysate. All medium was supplemented with 0. 5 g·L -1 L-glutamine,and adjusted to pH 5. 8.
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