对159份欧洲黑杨种质资源的生长、育种值、材性、养分利用率和水分利用率等13个性状进行分析，采用13种表型选优的抽样方法构建欧洲黑杨优质核心种质库。表型值评价表明：当抽样群体比例达到21.4%以上即能满足构建核心种质要求，而只有抽样群体比例达到30.2%以上 SSR 分子遗传多样性才能满足构建核心种质要求。最终确定的欧洲黑杨优质核心种质库包含48份种质资源，占原群体的30.2%；与原群体相比，均值和方差不存在显著差异，极差符合率和变异系数变化率分别为97.07%和89.72%；优质核心种质库的有效等位基因数、Nei’s 遗传多样性系数和 Shannon’s 信息指数与原群体之间没有显著差异，保持了原群体的遗传多样性。
A Preliminary Study on Construction of High-Quality Core Collection of Populus nigra
In this study,we analyzed 13 traits of 159 Populus nigra germplasm such as growth,breeding value,material properties,efficiency of water use efficiency and efficiency of nutrient utilization. A high-quality core collection of P. nigra was constructed by utilizing 13 kinds of sampling methods of phenotypic selection. Phenotypic value evaluation showed that the sampling rate reached to more than 21. 4% can meet the requirement of building core collection. However,the proportion of the sample population over 30. 2% was required for building the core collection in terms of SSR molecular genetic diversity. Ultimately,the determined high-quality core collection of P. nigra contained 48 germplasm resources,accounting for 30. 2% of the original germplasm. Compared with the original germplasm,there were no differences in the aspects of the mean and the variance,and the coincidence rate of range and the changeable rate of coefficient of variation were 97. 07%and 89. 72%. The high-quality core collection was no significantly different from the original group in the aspects of the effective number of alleles,Nei’s genetic diversity coefficient and Shannon’s information index,suggesting that the high-quality core collection should maintain the genetic diversity of the original group.
Populus nigrahigh-quality core collectionsampling methodgenetic diversity
国家科技期刊平台
NSTL
万方数据
维普
