Chemical Constituents in Rhizospheric Soil Extracts of Pinus massoniana and Liquidambar formosana
Objective]The differences of chemical constituents and content of rhizospheric soil extracts between Pinus massoniana and Liquidambar formosana were studied to understand the allelopathy mechanism of the species,and to provide basic data for further studies in chemical ecology of the species. [Method]Rhizospheric soil samples of P. massoniana and L. formosana were collected from pure forest of P. massoniana and mixed forest of L. formosana-Schima superba for extraction and separation of the chemical constituents using ethanol and ethylacetate. The chemical constituents of rhizospheric soils were analyzed by GC-MS, and their relative contents were determined by area normalization.[Result]In P. massoniana rhizospheric soil,109 compounds were identified from ethanol extraction with 6 compounds’ content > 2% ,and 56 compounds were identified from ethylacetate extraction with ,14 compounds’content >2%. The relative content of dehydroabitetic acid was the highest,accounting for 28. 98% of the ethanol extraction and 20. 36%of the ethylacetate extraction. The common organics of the two extractions from rhizospheric soil were dehydroabitetic acid and octadecanoic acid. Accordingly,96 and 63 compounds were identified from the ethanol and ethylacetate extraction of L. formosana rhizospheric soil,each with 8 and 9 compounds more than 2% in relative content. The relative content of octadecanoic acid and lupeol were the highest,accounting for 21. 81% of the ethanol extraction and 19. 01% of the ethylacetate extraction. The common organics of the two extractions from rhizospheric soil were octadecanoic acid and Phenol,2,4-bis (1,1-dimethylethyl). Moreover,the common organics of ethanol extraction from P. massoniana and L. formosana rhizospheric soil were dehydroabitetic acid,octadecanoic acid and stigmasterol,22,23-dihydro. The relative contents of dehydroabitetic acid and octadecanoic acid were the highest, accounting for 29. 98% and 21. 81%, respectively. The common organics of ethylacetate extraction were octadecanoic acid,n-hexadecanoic acid,phenol,2,4-bis (1,1-dimethylethyl) and stigmast-4-en-3-one. The relative contents of dehydroabitetic acid and lupeol were the highest for P. massoniana and L. formaosana,accounting for 20. 36% and 19. 01% of the total compounds respectively.[Conclusion]The ethanol extraction of rhizospheric soil from the two tree species were higher than the ethylacetate extraction in terms of the number of chemicals constitutes. The contents of organic acids were the highest,and that from P. massoniana was significantly higher than that from L. formosana. The major components of P. massoniana rhizospheric soil extraction were dehydroabitetic acid,octadecanoic acid and hexadecanoic acid. Dehydroabitetic acid content was the highest,accounting for 28. 98% of the total of all chemical constituents,7 times higher in P. massoniana than in L. formosana.
国家科技期刊平台
NSTL
万方数据
维普
