香榧藻斑病病原的分离鉴定及防治药剂筛选
Isolation and Identification of Pathogens of Algal Spots of Torreya grandis cv.'Merrillii'and Screening of the Algicides
刘艾涛 1叶碧欢 2陈友吾 2宋其岩 2李海波 2沈建军 2张昕3
作者信息
- 1. 浙江农林大学林业与生物技术学院 杭州 311300;浙江省林业科学研究院森林保护所 杭州 310023
- 2. 浙江省林业科学研究院森林保护所 杭州 310023
- 3. 浙江农林大学林业与生物技术学院 杭州 311300
- 折叠
摘要
[目的]探明香榧藻斑病的病原种类及其生物学特性,筛选对香榧藻斑病具有良好防治效果的药剂,为香榧藻斑病防治提供科学依据.[方法]在浙江省香榧主产区采集藻斑病病叶,结合传统形态学方法与分子生物学手段对从病叶中分离获得的香榧绿藻进行鉴定,明确病原藻的生物学特性,通过室内毒力测定和林间防治试验筛选防治香榧藻斑病的优良药剂.[结果]1)从香榧藻斑病病叶中分离获得 3种病原绿藻类群,结合藻株形态学特点以及SSU-ITS区间序列分析,3种绿藻类群分别被鉴定为链带藻、软克里藻和Tritostichococcus corticulus,均为香榧病原新记录种.3种香榧绿藻的培养条件相似,均适宜在初始pH 11、温度 25℃、光照/黑暗为 12 h/12 h光周期下的BG-11培养基中生长.2)以混合藻为靶标进行室内毒力测定试验显示,12%松脂酸铜悬浮剂灭藻效果最好,EC50 可达 8.33 mg·L-1,显著低于其他药剂(P<0.05),其后依次为 33.5%喹啉铜悬浮剂、80%波尔多液、20%噻菌铜悬浮剂和 45%石硫合剂.林间防治试验表明,施药 30天后,12%松脂酸铜和 33.5%喹啉铜悬浮剂的防治效果分别达 78.11%和 71.62%.[结论]从浙江省香榧上新发现 3种病原绿藻类群,藻群生物学特性类似.室内毒力测定和林间防治试验表明,12%松脂酸铜和33.5%喹啉铜悬浮剂对香榧藻斑病有较好防治效果.
Abstract
[Objective]The study aims to explore the pathogens of algal spots of Torreya grandis cv.'Merrillii'and their biological characteristics,as well as screen efficient algicides,so as to provide scientific basis for controlling the disease.[Method]The diseased leaves of T.grandis cv.'Merrillii'were collected from the main planting areas in Zhejiang Province,and the algae were isolated from diseased leaves,and then identified by traditional morphological observation combined with molecular biological methods.The biological characteristics of the pathogenic algae were studied,and efficient algicides for controlling the disease were screened by laboratory virulence assay and the field control experiment.[Result]1)Three groups of synantectic pathogenic algae were isolated from algal spots,and they were identified as Desmodesmus armatus,Klebsormidium flaccidum and Tritostichococcus corticulus,respectively,based on the morphological characteristics and the SSU-ITS sequence analysis,all of which were newly reported pathogens of T.grandis cv.'Merrillii'.The culture conditions of the three alga species were similar,and they all grew well in BG-11 medium at 25℃with an initial pH of 11 and a photoperiod of 12 h light/12 h dark.2)With the mixed algae as the target,a laboratory toxicity assay showed that 12%copper rosinate suspension had the best algal killing effect,and the EC50 was 8.33 mg·L-1,which was significantly lower than other algicides(P<0.05),followed by 33.5%copper quinoline suspension agent,80%Bordeaux mixture,20%thiophanate copper suspension agent and 45%lime sulfur mixture.The field control test showed that after 30 days of application,the control effects of 12%copper rosinate and 33.5%copper quinoline suspending agent were 78.11%and 71.62%,respectively.[Conclusion]Three pathogenic green algae have newly been discovered from T.grandis cv.'Merrillii'in Zhejiang Province,and the biological characteristics of the three alga species are similar.Laboratory toxicity assay and field control test show that 12%copper rosinate and 33.5%copper quinoline suspending agent have good control effect on algal spot of T.grandis cv.'Merrillii'.
关键词
香榧/藻斑病/鉴定/生物学特性/毒力测定/林间防治Key words
Torreya grandis cv.'Merrillii'/algal spot/identification/biological characteristics/toxicity test/field control引用本文复制引用
基金项目
浙江省省院合作林业科技项目(2019SY09)
浙江省省属科研院所扶持专项(2021F1065-2)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据