Cellulose is deemed as the most abundant and renewable resource on the earth.Enzymatic saccharification of cellulose to glucose is an ideal bioconversion,but its low efficiency significantly impedes the development of cellulose industrialization.Recognition and deconstruction of different recalcitrant factors and improving enzymatic hydrolysis efficiency are still the vital scientific problems in the high valorization of cellulose.In this article,various recalcitrant factors that impeding the enzymatic hydrolysis were summarized at multidimensional levels,in the aspects of macrostructure,microstructure,ultra-structure,molecule,chemical group,chemical element,chemical bonds and gene.Furthermore,we revealed that recalcitrant factors exhibited typical heterogeneity in specific biomass,high dynamics in different development stage of plant,and complex relation during the pretreatment.Finally,several novel research directions and strategy on recalcitrant barrier were proposed:cell wall interface recalcitrance,modification and valorization of cell wall from a biorefinery concept and saccharification hydrolysis with synergies of multiple enzymes.
国家科技期刊平台
NSTL
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