Regulation of Lactarius akahatsu on the Growth and Root Metabolites of Pinus massoniana and Pinus armandii Seedlings
[Objective]The study is aimed to investigate the effects of Lactarius akahatsu inoculation on the growth,root development,and metabolites of Pinus massoniana and Pinus armandii seedlings,and to provide a scientific basis to cultivate pine mycorrhizal seedlings and symbiotic cultivation of Lactarius akahatsu.[Method]Potted seedlings of Pinus massoniana and Pinus armandii were placed in the greenhouse and applied with two treatments of Lactarius akahatsu inoculation and non-inoculation,the difference in the mycorrhizal colonization rate and mycorrhizal dependency,shoot and root growth indicators,the root architecture traits(total root length,root surface area,root volume,root tips,bifurcation),the root morphology traits(specific root length,root tissue density,specific root surface area)and main root metabolites were studied.[Result]Evident mycorrhizal morphology of the Lactarius akahatsu was formed in the seedlings of the two pines,and displayed a high mycorrhizal colonization rate and mycorrhizal dependency.The inoculation of Lactarius akahatsu promoted the growth of seedlings to a certain extent,but significant differences were found between the two species.Lactarius akahatsu inoculation significantly increased the plant seedling's height and dry aboveground weight of Pinus massoniana seedlings,whereas the plant height and dry underground weight of Pinus armandii.The total root length,root surface area,root volume,root tip number,and bifurcation of the two pine species increased by Lactarius akahatsu inoculation,while root vigor and cation exchange capacity decreased.Inoculation with Lactarius akahatsu significantly affected the content of main metabolites in the roots of the two pine species.After Lactarius akahatsu inoculation,the total phenols content and total flavonoid content in the roots of Pinus massoniana seedlings decreased,while the total triterpenoid content,total alkaloid content and total sterol content increased.The trends in metabolites in the root of Pinus armandii seedlings was opposite to that of Pinus massoniana seedlings.[Conclusion]Lactarius akahatsu inoculation improves the root morphology and biomass of the seedlings,and affects the content of main metabolites in the roots,and therefore Lactarius akahatsu inoculation is feasible to cultivate pine mycorrhizal seedlings and symbiotic cultivation of ectomycorrhizal fungi.
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