Establishment of Tissue Culture Systems for Inducing Adventitious Buds from Leaves of Two Deutzia Species
[Objective]Seeds of Deutzia are small,and their survival rate is low.Because the branches of Deutzia are hollow,branch propagation is difficult.This study aims to establish a complete tissue culture system through the induction of adventitious buds from the leaves of plants in the genus Deutzia,and lay the foundation for the genetic transformation system and molecular breeding.It also provides theoretical basis and practical guidance for the innovation of de novo domestication of wild Deutzia.[Method]In this study,branches with leaves of D.parviflora and D.baroniana,two common species of Deutzia in the Qinling Mountains of Shaanxi Province,were used as explants.The explants were used for primary culture,subculture,rooting culture,and then,the leaves of the cultured plantlets were used to induce callus and adventitious buds.[Result]The optimal disinfection method of explants was to disinfect the surface with 75%alcohol for 10s and 0.1%mercuric chloride for 3 min.The survival rate was 100%and the contamination rate was 0.The optimal medium for D.parviflora subculture was MS+2 mg·L-1 6-BA,and the proliferation coefficient was 2.8.The optimal medium for D.baroniana subculture was MS+5 mg·L-1 6-BA-1,and the proliferation coefficient was 4.53.The optimal medium for rooting culture was 1/2 MS+0.8 mg·L-1 IBA,with the proliferation coefficient of 1.74 for D.parviflora and 7.86 for D.baroniana.The explants for callus induction were the leaves on the 2-4 nodes from top to bottom of 30 day old root seedlings.The optimal medium for D.parviflora leaves to induce callus was WPM+1 mg·L-1 2,4-D,with the induction rate of 63%.The optimal medium for D.baroniana leaves to induce callus was WPM+0.1 mg·L-1 2,4-D,and the induction rate was 70%.[Conclusion]This study has successfully established a regeneration system for adventitious shoot induction and plant formation using the leaves of D.parviflora and D.baroniana as explants.The optimal medium for D.parviflora adventitious bud differentiation from leaves was MS+2 mg·L-1 6-BA+0.3 mg·L-1 IBA.The optimal medium for the adventitious bud differentiation from D.baroniana leaves was MS+5 mg·L-1 6-BA+0.05 mg·L-1 NAA.
Deutziaadventitious budshade-tolerantshrubsde novo domestication
万方数据
维普
