2种溲疏叶片不定芽诱导的组织培养体系构建

Establishment of Tissue Culture Systems for Inducing Adventitious Buds from Leaves of Two Deutzia Species

王亚鑫 ¹尚鑫 ¹郝英男 ¹宁小艺 ¹马跃 ¹甘思楠 ¹李进宇 ²张鑫¹

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作者信息

1. 西北农林科技大学林学院杨凌 712100
2. 北京市园林绿化科学研究院北京 100102
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摘要

[目的]通过溲疏属植物叶片诱导不定芽,建立完整的组织培养体系,为构建溲疏属植物遗传转化体系和分子育种提供参考,也为从头驯化野生溲疏属植物种质资源的创新提供理论依据和实践指导.[方法]以 2种陕西秦岭常见野生溲疏属植物(小花溲疏和钩齿溲疏)的带叶茎段作为外植体,通过初代培养、继代培养和生根培养建立其快速繁殖体系,再利用培养植株的叶片诱导愈伤组织和不定芽诱导的方式建立其叶片诱导不定芽体系.[结果]外植体最佳消毒方式为 75%乙醇表面消毒 10 s,0.1%升汞表面消毒 3 min,其成活率为 100%,污染率为 0.小花溲疏丛生芽增殖最佳的培养基配方为MS+2 mg·L-1 6-BA,其增殖系数为 2.8.钩齿溲疏丛生芽最佳培养基配方为MS+5 mg·L-1 6-BA,其增殖系数为 4.53.生根培养最佳的培养基配比为 1/2 MS+0.8 mg·L-1 IBA,其小花溲疏的生根系数为 1.74,钩齿溲疏的生根系数为 7.86.选择 30天生根苗从上至下第 2～4节叶片作为诱导愈伤组织的外植体材料.小花溲疏叶片诱导愈伤组织最佳的培养基配比为WPM+1 mg·L-1 2,4-D,其诱导率为 63%,钩齿溲疏叶片诱导愈伤组织最佳的培养基配比为WPM+0.1 mg·L-1 2,4-D,其诱导率为 70%.[结论]以小花溲疏和钩齿溲疏的叶片作为外植体,成功建立了诱导不定芽形成和植株再生体系.小花溲疏叶片不定芽分化最佳的培养基配比为MS+2 mg·L-1 6-BA+0.3 mg·L-1 IBA,钩齿溲疏叶片不定芽分化最佳的培养基配比为MS+5 mg·L-1 6-BA+0.05 mg·L-1 NAA.

Abstract

[Objective]Seeds of Deutzia are small,and their survival rate is low.Because the branches of Deutzia are hollow,branch propagation is difficult.This study aims to establish a complete tissue culture system through the induction of adventitious buds from the leaves of plants in the genus Deutzia,and lay the foundation for the genetic transformation system and molecular breeding.It also provides theoretical basis and practical guidance for the innovation of de novo domestication of wild Deutzia.[Method]In this study,branches with leaves of D.parviflora and D.baroniana,two common species of Deutzia in the Qinling Mountains of Shaanxi Province,were used as explants.The explants were used for primary culture,subculture,rooting culture,and then,the leaves of the cultured plantlets were used to induce callus and adventitious buds.[Result]The optimal disinfection method of explants was to disinfect the surface with 75%alcohol for 10s and 0.1%mercuric chloride for 3 min.The survival rate was 100%and the contamination rate was 0.The optimal medium for D.parviflora subculture was MS+2 mg·L-1 6-BA,and the proliferation coefficient was 2.8.The optimal medium for D.baroniana subculture was MS+5 mg·L-1 6-BA-1,and the proliferation coefficient was 4.53.The optimal medium for rooting culture was 1/2 MS+0.8 mg·L-1 IBA,with the proliferation coefficient of 1.74 for D.parviflora and 7.86 for D.baroniana.The explants for callus induction were the leaves on the 2-4 nodes from top to bottom of 30 day old root seedlings.The optimal medium for D.parviflora leaves to induce callus was WPM+1 mg·L-1 2,4-D,with the induction rate of 63%.The optimal medium for D.baroniana leaves to induce callus was WPM+0.1 mg·L-1 2,4-D,and the induction rate was 70%.[Conclusion]This study has successfully established a regeneration system for adventitious shoot induction and plant formation using the leaves of D.parviflora and D.baroniana as explants.The optimal medium for D.parviflora adventitious bud differentiation from leaves was MS+2 mg·L-1 6-BA+0.3 mg·L-1 IBA.The optimal medium for the adventitious bud differentiation from D.baroniana leaves was MS+5 mg·L-1 6-BA+0.05 mg·L-1 NAA.

关键词

溲疏属/不定芽/耐荫/灌木/从头驯化

Key words

Deutzia/adventitious bud/shade-tolerant/shrubs/de novo domestication

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基金项目

北京市园林绿化研究院绿化植物育种项目(YZQN202301)

北京市重点实验室课题(K4050423193)

北京市重点实验室课题(K4050422164)

北京市重点实验室课题(K4020121025)

出版年

2024

林业科学

中国林学会

林业科学

CSTPCDCSCD北大核心

影响因子：1.272

ISSN：1001-7488

参考文献量30

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