Cloning and Expression Level Study on Agouti Gene in Reddish-brown Wusuli Raccoon Dog and wild-type Raccoon Dog
In this study,wild-type and reddish-brown Wusuli raccoon dogs was selected for research object,respectively.The 395 bp DNA sequence of the Agouti was cloned.The polymorphism and homology of the Raccoon dogs,wild-type Wusuli raccoon dog and the other species was analyzed.The expression level of Agouti gene was compared in the two kinds of raccoon dog.The results showed that the homologous rate in canis familiaris,felis catus,chimpanzee,sus scrofa,equus caballus and Ovis aries was 99.49 ％,87.50 ％,86.47 ％,85.71％,85.82 ％,85.82 ％,respectively.The Agouti gene was high conservative in the other species.Comparing analysis revealed there is a base substitution from G to T which leads to the amino acid from tryptophan to glycine.A complete match was found in the two kinds of raccoon dog.Based on the independent-samples T test in SPSS software,the results showed that Agouti gene was stably expressed in of skintissue of reddish-brown Wusuli raccoon dog and the expression quantity of Agouti gene in reddish-brown Wusuli-raccoon dog was 1.09 times of that of wild-type Wusuli raccoon dog (F=(1+E)-△△Ct).However,there was no significant difference between them(P＞0.05).
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万方数据
维普
