Isolation and Screening of Endophytic Fungi of Oyster Against Alzheimer's Disease and Optimization of Fermentation Conditions
The endophytic fungi were isolated from wild oysters in Qingdao sea area by mycelial tip-cutting method,and identified by morphology and 18S rDNA,and categorized into species and genera.The re-sults showed that a total of 13 strains of fungi were isolated from oysters,and all of them were identified as belonging to Ascomycetes,among which 7 strains were Cladosporium,which is the dominant genus of oyster endophytic fungi and accounted for 53%of the total number of isolated fungi,in addition to two strains of Penicillium,and one strain each of Arthrinium,Lecanicillium,Alternaria,and Periconia.I-solated oyster endophytic fungi were screened for anti-Alzheimer's disease activity in vitro,and the fer-mentation products of five strains of the fungi possessed both acetylcholinesterase-inhibiting and β-amyloid(Aβ1-42)aggregation-inhibiting activities.For further development and utilization,the fermentation condi-tions of the ML3 strain were optimized by a one-way combined response surface methodology.The results showed that the optimal fermentation conditions were seed liquid inoculation of 10%at 28℃for 12 days.Under this condition,the inhibition rate of AChE and Aβ1-42 from secondary metabolites were 57.65%and 52.43%,respectively,which were 9.35%and 7.63%higher than those before the unoptimized,and the primary and secondary factors affecting the above two indexes were:seed liquor inoculation＞fermenta-tion temperature＞fermentation time.
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