Effects of Cow Manure Organic Fertilizer on Soil Microbial Communities
To explore effects of cow manure organic fertilizer on structure and function of soil microbial communities,fully decomposed and fermented cow manure was used as the treatment material.Two treatments were designed:the control group(CK)with no base fertilizer and the treatment group(DT)applying 29850 kg/hm2 of cow manure as base fertilizer.The organic matter content was determined by the potassium dichromate method,and structure and functional abundance of soil microbial communities were studied by the metagenomic sequencing technology.The results showed that the organic matter con-tent in soil significantly increased with the application of cow manure organic fertilizer.After the cow ma-nure treatment,there were more genes in soil,and the number of unique genes rose remarkably.The rela-tive abundance of microbial populations in soil changed at the kingdom,phylum,class,family,genus,and species levels.The cow manure treatment notably improved the relative abundance of Verrucomicrobi-ae,Thermomicrobia,Blastocatellia,Nitrososphaeria,Ktedonobacteria and Rubrobacteria,while signifi-cantly decreased the relative abundance of Nitrospira,Deltaproteobacteria,Gammaproteobacteria and Planctomycetia.PCA and NMDS analysis results further indicated that the structure of microbial commu-nities in soil changed with the application of cow manure organic fertilizer.The annotation results of CAZy,KEGG,and eggNOG databases showed that cow manure organic fertilizer promoted changes in functional abundance of soil microbial communities.In summary,cow manure organic fertilizer had a posi-tive impact on the effective use of farmland soil.
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