首页|基于高通量测序技术的核电厂温排水区表层细菌多样性分析

基于高通量测序技术的核电厂温排水区表层细菌多样性分析

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核电厂产生的温排水对附近海域的海水理化性质、微生物群落结构会产生一定影响.检测了海阳核电站排水口周围海水的理化性质,并通过高通量测序技术对浮游微生物的16S rRNA基因进行测序,分析温排水对周围海水浮游微生物细菌群落和功能的影响.结果发现,与正常区域海水相比:温排水区海水温度升高2.7℃,氧化还原电位升高6.8 mV,溶解氧含量较低;温排水区浮游微生物多样性增加,细菌群落组成中参与硝化作用的硝化螺旋菌门(Nitrospirae)相对丰度大幅降低;在属水平上,参与氨氮-亚硝酸盐氮-硝酸盐氮循环的科迪单胞菌属(Kordiimonas)相对丰度显著增加,硝化刺菌属(Nitrospina)与伍斯氏菌属(Woeseia)相对丰度显著降低;温排水区浮游微生物与代谢相关的基因相对丰度增加,代谢过程活跃.为进一步探究温排水对海洋生态的影响提供了依据.
Analysis of Surface Bacterial Diversity in Thermal Drainage Area of Nuclear Power Plant Based on High-Throughput Sequencing
Once-through warm water discharged from nuclear power plants has some effects on physical and chemical properties of seawater and microbial community structures of nearby sea areas.Physicochemical properties of seawater around Haiyang Nuclear Power Plant outlets were examined,and the 16S rRNA gene of plankton was sequenced using high-throughput sequencing to analyze effects of once-through warm water discharge on the communities and functions of plankton bacteria in surrounding seawater.Results showed that,compared with normal seawater,temperature of the thermal drainage areas increased by 2.7℃,the redox potential increased by 6.8 mV,while the dissolved oxygen content was lower.In the ther-mal drainage areas,the diversity of planktonic microorganisms improved,and the relative abundance of Nitrospirae involved in nitrification reduced significantly.In the genus level,the relative abundance of Kordiimonas participating in the ammonia nitrogen-nitrite nitrogen-nitrate nitrogen cycle increased no-tably,while the relative abundance of Nitrospina and Woeseia decreased remarkably.The relative abun-dance of genes related to metabolisms of planktons increased with active metabolic process in thermal drainage areas.It provides a basis for further research on influences of once-through warm water discharge on marine ecology.

once-through warm watermicrobiologyhigh-throughput sequencingphysicochemical prop-erties

韩涛、王有军、潘万松、李怡、姚岚、沈钰博、刘云财、赵明波

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山东核电有限公司,山东 烟台 265116

威海市蓝色经济研究院有限公司,山东 威海 264400

温排水 微生物 高通量测序 理化性质

2024

青岛农业大学学报(自然科学版)
青岛农业大学

青岛农业大学学报(自然科学版)

影响因子:0.37
ISSN:1674-148X
年,卷(期):2024.41(3)