Analysis of Surface Bacterial Diversity in Thermal Drainage Area of Nuclear Power Plant Based on High-Throughput Sequencing
Once-through warm water discharged from nuclear power plants has some effects on physical and chemical properties of seawater and microbial community structures of nearby sea areas.Physicochemical properties of seawater around Haiyang Nuclear Power Plant outlets were examined,and the 16S rRNA gene of plankton was sequenced using high-throughput sequencing to analyze effects of once-through warm water discharge on the communities and functions of plankton bacteria in surrounding seawater.Results showed that,compared with normal seawater,temperature of the thermal drainage areas increased by 2.7℃,the redox potential increased by 6.8 mV,while the dissolved oxygen content was lower.In the ther-mal drainage areas,the diversity of planktonic microorganisms improved,and the relative abundance of Nitrospirae involved in nitrification reduced significantly.In the genus level,the relative abundance of Kordiimonas participating in the ammonia nitrogen-nitrite nitrogen-nitrate nitrogen cycle increased no-tably,while the relative abundance of Nitrospina and Woeseia decreased remarkably.The relative abun-dance of genes related to metabolisms of planktons increased with active metabolic process in thermal drainage areas.It provides a basis for further research on influences of once-through warm water discharge on marine ecology.