This study aimed to assess the prevalence of avian leukosis virus(ALV)in a local chicken breed from Longyan and to analyze the molecular characteristics of the circulating strains.Suspected ALV-infected chickens were diagnosed using necropsy,histopathological examination,and reverse transcription polymerase chain reaction(RT-PCR).The gp85 gene of the virus was amplified and sequenced for further analysis.Results indicated that infected chickens presented with hepatomegaly and cardiomegaly,along with pinpoint petechial hemorrhages on organ surfaces and marked ascites.There was a significant presence of lymphocytic foci within the internal organs.The gp85 gene sequence of the isolated strain showed a high degree of homology with the reference strain of the J subgroup,with a sequence identity of 87.8％to the prototype strain HPRS-103.Phylogenetic analysis placed this strain within the same subclade as the HN0001 reference strain of the J subgroup.The findings of this study provide valuable insights for monitoring the epidemiological trends of ALV in local chicken farms in Longyan,formulating effective control strategies,and understanding the genetic evolution of the virus.
local chicken breedavian leukosisgp85 geneetiological analysis
维普
万方数据
