首页|Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl
Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl
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To determine the optimal embryogenic capacity (somatic embryo production) of the selected elite nematode-resistant genotypes of Pinus thunbergii, variables such as embryogenic tissue (ET) morphology, maternal genotype, proliferation rate and tissue age were analyzed. ET mor-phology and histological evaluation of the proliferation stage showed a decrease in filamentous clump and protuberant surfaces and a decline in the acetocarmine-staining area, which indicates a decrease in somatic embryo production (SEP). Variations in cell physiology during the prolifera-tion stage showed that SEP was positively correlated with soluble sugars and proteins, but negatively correlated with starch, peroxidase, and superoxidase. In addition, SEP was significantly (p < 0.001) affected by maternal genotype, tis-sue age and proliferation rate. Moreover, SEP was positively correlated with proliferation rate (r = 0.98, p < 0.001), but negatively correlated with tissue age (r = ? 0.95, p < 0.001). In general, the results suggest that SEP could be assessed in ET proliferation stages by the apparent cell morphology, histology, proliferation rate and tissue age, which provides novel insights for evaluating the ET maturation capacity (number of somatic embryos) during the proliferation stage of P. thunbergii somatic embryogenesis.
Tingyu Sun、Yanli Wang、Lihua Zhu、Xiaowei Liu、Qingtong Wang、Jianren Ye
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College of Forestry,Nanjing Forestry University, Nanjing 210037,Jiangsu,People's Republic of China
Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing Forestry University, Nanjing 210037,Jiangsu,People's Republic of China
Collaborative Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University, Nanjing 210037,Jiangsu,People's Republic of China
国家重点研发计划国家重点研发计划Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)Doctorate Fellowship Foundation of Nanjing Forestry University
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