首页|Method to measure tree-ring width,density,elemental composition,and stable carbon and oxygen isotopes using one sample
Method to measure tree-ring width,density,elemental composition,and stable carbon and oxygen isotopes using one sample
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Tree-ring width(RW),density,elemental com-position,and stable carbon and oxygen isotope(δ13C,δ18O)are widely used as proxies to assess climate change,ecology,and environmental pollution;however,a specific pretreat-ment has been needed for each proxy.Here,we developed a method by which each proxy can be measured in the same sample.First,the sample is polished for ring width meas-urement.After obtaining the ring width data,the sample is cut to form a 1-mm-thick wood plate.The sample is then mounted in a vertical sample holder,and gradually scanned by an X-ray beam.Simultaneously,the count rates of the fluorescent photons of elements(for chemical characteriza-tion)and a radiographic grayscale image(for wood density)are obtained,i.e.the density and the element content are obtained.Then,cellulose is isolated from the 1-mm wood plate by removal of lignin,and hemicellulose.After pro-ducing this cellulose plate,cellulose subsamples are sepa-rated by knife under the microscope for inter-annual and intra-annual stable carbon and oxygen isotope(δ13C,δ18O)analysis.Based on this method,RW,density,elemental com-position,δ13C,and δ18O can be measured from the same sample,which reduces sample amount and treatment time,and is helpful for multi-proxy comparison and combination research.
Tree-ring widthTree-ring densityTree-ring elemental compositionTree-ring stable carbon and oxygen isotopes
Key Laboratory of Cenozoic Geology and Environment,Institute of Geology and Geophysics,Chinese Academy of Sciences,Beijing 100029,People's Republic of China
CAS Center for Excellence in Life and Paleoenvironment,Beijing 100044,People's Republic of China
College of Earth Sciences,University of Chinese Academy of Sciences,Beijing 100049,People's Republic of China
Graduate School of Environment Studies,Nagoya University,Nagoya 4648601,Japan
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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaStrategic Priority Research Program of the Chinese Academy of Sciences,ChinaKey Research Program of the Institute of Geology and GeophysicsCAS Youth Interdisciplinary Team