Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,Bursaphelenchus xylophilus

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外文摘要：In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be downregulated.In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system.Here in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite effect.In addition,RNAi of sft-4 changed the female-male ratio and lifespan of PWN.In bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly weakened.These results indicate that the influence of sft-4 on PWN pathogenicitymay be mainly through regulating reproductive function of PWN and its lifespan.

外文关键词：

Black pinePinaceaeBursaphelenchus xylophilusSft-4In situ hybridizationRNAiPathogenicity

作者：

Shuisong Liu、Linsong Wang、Ronggui Li、Mengyu Chen、Wenjun Deng、Chao Wang、Guicai Du、Qunqun Guo、Tao Xu

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作者单位：

College of Life Sciences,Qingdao University,Qingdao 266071,People's Republic of China

基金：

Shandong Provincial Natural Science Foundation,ChinaQingdao Municipal Peoplebenefitting Demonstration Project of Science and Technology,China

项目编号：

ZR2020MC12323-2-8-cspz-8-nsh

出版年：

2024

DOI：

10.1007/s11676-023-01687-2

林业研究(英文版)

东北林业大学,中国生态学学会

林业研究(英文版)

CSTPCDEI

影响因子：0.365

ISSN：1007-662X

年,卷(期)：2024.35(5)