COMPARATIVE STUDY ON HPLC FINGERPRINT OF TRIPTERYGIUM WILFORDII BEFORE AND AFTER BEING PROCESSED WITH FRESH SHEEP BLOOD
Objective To explore the mechanism of detoxification of tripterygium wilfordii processed with fresh sheep blood.Methods Agilent Eclipse XDB-C18 column was used as the chromatographic col-umn,acetonitrile and 0.1%phosphoric acid solution were used as mobile phase,gradient elution was per-formed at the flow rate of 0.4 mL/min,and the detection wavelength was 230 nm.The similarity evalua-tion system of TCM chromatographic fingerprint and SPSS17.0 statistical software were used to analyze the difference in the fingerprint of Tripterygium wilfordii before and after being processed with sheep blood,and the changes of its components were observed.Results The HPLC fingerprints of tripterygium wilfordii before and after being processed with sheep blood were established.The content of 6 components including tripterygium erythrodin decreased after being processed with sheep blood.Conclusion The estab-lished fingerprint of Tripterygium wilfordii has good reproducibility and can accurately reflect the changes of composition of tripterygium wilfordii before and after being processed with sheep blood.
万方数据
维普
