Objective To explore the treatment methods for children with AL.Methods The Ficoll Hypaque method was used to isolate MNCs from AL patients,who were divided into four groups.Control group:cultured in RPMI1640 medium containing 10%FCS;Experiment 1 group:using MDP alone;Ex-periment 2 group:rhGM-CSF+rhIL-4+rhTNF-α;Experiment 3 group:rhGM-CSF+rhIL-4+rhTNF-α+MDP.Observe daily cell growth and morphology,count the number of cells in each group after 8 days of culture,and use flow cytometry to detect the immune phenotype of each group of cells.Results After 3 days of cultivation in the control group,a large number of cells died and the quantity significantly de-creased;And all experimental groups showed changes in cell morphology from day 2 to day 8 of cultiva-tion;The number of cells and the proportion of HLA DR,CD1a,and CD83 cells in each experimental group were higher than those in the control group,with the highest in experimental group 3(P<0.05).Conclusion MDP has an amplifying effect on bone marrow DCs in children with AL and promotes their maturation,with a stronger synergistic effect with cytokines.
维普
万方数据
