EFFECT OF MDP ON THE CULTURE AND PROLIFERATION OF DC DERIVED FROM BONE MARROW IN CHILDREN WITH AL IN VITRO
Objective To explore the treatment methods for children with AL.Methods The Ficoll Hypaque method was used to isolate MNCs from AL patients,who were divided into four groups.Control group:cultured in RPMI1640 medium containing 10%FCS;Experiment 1 group:using MDP alone;Ex-periment 2 group:rhGM-CSF+rhIL-4+rhTNF-α;Experiment 3 group:rhGM-CSF+rhIL-4+rhTNF-α+MDP.Observe daily cell growth and morphology,count the number of cells in each group after 8 days of culture,and use flow cytometry to detect the immune phenotype of each group of cells.Results After 3 days of cultivation in the control group,a large number of cells died and the quantity significantly de-creased;And all experimental groups showed changes in cell morphology from day 2 to day 8 of cultiva-tion;The number of cells and the proportion of HLA DR,CD1a,and CD83 cells in each experimental group were higher than those in the control group,with the highest in experimental group 3(P<0.05).Conclusion MDP has an amplifying effect on bone marrow DCs in children with AL and promotes their maturation,with a stronger synergistic effect with cytokines.
万方数据
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