Construction of an expression vector of ICE1 gene of Brassica campestris and its transformation into tobacco
A complete open reading frame of ICE1 gene was cloned from Brassica campestris (rape).Then the ICE1 gene expression vectors containing CaMV35S promoters were constructed.The ICE1 gene was introduced into tobacco genome by Agrobacterium tumefaciens mediated transformation.The PCR assay results showed that ICE1 had been integrated into the genome of tobacco successfully.When transgenic and non-transgenic plants were stressed by 2 ℃ with seven days,the non-transgenic tobacco wilted seriously but the transgenic tobacco underwent little change and the content of MDA was significantly higher in the non-transgenic plants than in the transgenic lines.When transgenic and non-transgenic plants were stressed at 0,-2,-4 ℃ for 1 h,APX and SOD activities were significantly higher in the transgenic lines than in the non-transgenic plants.
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