To investigate the effect of iron ion concentration on Pseudomonas aeruginosa,the growth state of P.aeruginosa reference strain(PAO1)was first explored under the conditions of iron deficiency and iron-rich,and the results showed that iron deficiency restricted the growth of PAO1.In order to explore this reason,the differentially-expressed proteins of P.aeruginosa PAO1 under different iron ion conditions were detected using proteomic mass spectrometry,and the data indicated that the expression of phenazine proteins related to the synthesis of pyocyanin was significantly upregulated under iron defi-ciency conditions.The quantitative detection results of pyocyanin further verified that the amount of pyocyanin produced by P.aeruginosa PAO1 in the iron-deficient environment was signifiicantly in-creased compared with that in an iron-rich environment.By detecting the effect of exogenous addition of pyocyanin on the growth state of P.aeruginosa PAO1 under iro-deficient and iron-rich conditons,the data showed that pyocyanin could lead to a slow growth of P.aeruginosa PAO1 in the early stage of growth and a significant decrease in bacterial density in the middle stage.The results showed that the change in the growth state of P.aeruginosa might be due to the killing effect of pyocyanin produced by P.aeruginosa PAO1 under iron-deficient conditions
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