The mouse S180 tumor cells treated with polysaccharide LDS-1,mannatide and blank control were used as research materials.The data were sequenced via RNA-Seq and analyzed by the bioinformat-ics method.The results showed that 8.27,8.35 and 8.97 G of the analytical data were obtained in the LDS-1,mannan peptide and blank control group respectively.Analysis of highly expressed genes(FPKM＞2 200)showed that LDS-1 regulated cell division mainly by down-regulating Rplp1 gene and inhibited the division of S180 cells in G1 phase.A differential gene analysis showed that LDS-1 inhibited the mi-gration of S180 tumor cells mainly by up-regulating the expression of Alb,Timp4 and Apoa1 gene regula-tory proteins or cell adhesion.Kyoto encyclopedia of genes and genomes pathway enrichment results showed that the key genes of LDS-1 inhibiting S180 tumor growth were mainly enriched in the peroxi-some proliferator activated receptor(PPAR)signaling pathway.Fabp3 gene in the upstream of this path-way was down-regulated and several genes such as Apoa1,Slc27a2 and Adipoq were up-regulated in the downstream,which regulated lipid metabolism and cholesterol metabolism of S180 cells.It was found that LDS-1 could inhibit the proliferation,division,energy acquisition,migration and invasion of S180 tumor cells through regulating the high expression of key genes Rplp1,differential expression of key genes Alb,Timp4 andApoal,and the PPAR signaling pathway.
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