A sequencing and bioinformatic analysis of the Double B-box(DBB)gene family of Lycium ruthenicum were carried out in order to provide reference for further study on the function of LrDBB genes.The DBB gene family members of Lycium ruthenicum were identified and analyzed via the bioin-formatic method based on the transcriptome database of Lycium ruthenicum under NaCl treatment.A total of 32 DBB family members were selected from the relevant transcriptome data,which were divided into 5 subfamilies.In the prediction of protein subcellular localization,the family members were mainly located in the nucleus.The molecular weight of the DBB family proteins ranged from 5 156.83 to 53 308.44 D;the isoelectric point from 4.58 to 8.96;the total atomic number was between 692 and 7 324;the number of amino acids ranged from 46 to 476 aa and the lipid solubility index from 51.06 to 92.88.All of the DBB proteins were hydrophilic and unstable proteins on most of the protein members.The N-terminal of LrDBB proteins had a B-box conserved domain,and random curling and α-helix occupied a large pro-portion.In the expression pattern,it was found that members of the LrDBB gene family exhibited high expression levels in both the roots and leaves of NaCl stressed plants.Under salt stress,there were sig-nificant differences in the expression level of the DBB genes in different organs of Lycium ruthenicum,suggesting that this family of genes might play a regulatory role in salt stress.
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