Study on the Adsorption and Purification Characteristics and Antioxidant Activity of Tea Saponins by Macroporous Adsorption Resin
Tea saponins have various biological activities and can be applied in various fields.However,at present,the extracted tea saponins generally have low purity,therefore they are not applicable for further development and utilization,so the isolation and purification are required.This study selected the resin with the best performance in purifying tea saponins from 18 types of macroporous adsorption resins,analyzed its adsorption isotherms,adsorption thermodynamics and adsorption kinetics characteristics,and the difference of antioxidant activity between before and after purification was investigated.The results showed that the kind of X-5 macroporous adsorption resin had the best purification effect among the 18 selected macroporous adsorption resins,with the yield of(84.1±0.07)％;The adsorption thermodynamics of tea saponins can be better fitted by the Langmuir model(R2＞0.99),compared with Freundlich model;The adsorption process of tea saponins by the kind of X-5 macroporous adsorption resin belonged to spontaneous,entropy reducing,and exothermic physical adsorption.Furthermore,it is more suitable to use the quasi second-order kinetic equation(R2＞0.99)for fitting;After purification by X-5 macroporous adsorption resin,the purity of tea saponins increased from(24.18±0.3)％to(80.62±0.42)％;The ability of purified tea saponins to scavenge ABTS free radicals,DPPH free radicals,and total reducing power increased to 1.63 times,1.61 times,and 1.57 times,respectively,before purification,which indicated that the purification effect was significant.
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