Effects of Exogenous CaCl2 on Cotton Seed Germination and Antioxi-dant Enzymes Activities of Seedling Leaves under Salt Stress
To explore the effects of exogenous calcium chloride(CaCl2)initiator on cotton seed germination,seedlings growth and antioxidant enzymes activities of leaf under salt stress,'Tianyun 0769'was used as acotton variety for testing material.The experi-ment was carried out fiter paper that seeds could germinate on it.With the conditions of salt concentration(NaCl,150 mmol/L)and calcium chloride concentration(CaCl2,0,10,20,30,40,50 mmol/L),the effects of exogenous CaCl2 on cotton seed germination index(GP,GR,GI,VI),seedling biomass,electrolyte exudation rate and activities of antioxidant enzymes(SOD,POD,CAT and APX)under salt stress were studied.The research showed that cotton seed germination index(GP,GR,GI,VI),the dry mass and fresh mass of cotton seedlings,the activities of antioxidant enzymes(SOD,POD,CAT and APX)decreased significantly compared with CK(P＜0.05)under 150mmol/L NaCl stress,the electrolyte leakage rate increased under the same other conditions.With the increase of CaCl2 concentration,cotton seed germination indexes(GP,GR,GI,VI),the dry mass and fresh mass of cotton seedlings,activities of antioxidant enzymes(SOD,POD,CAT and APX)were firstly increased and then decreased,the electrolyte exudation rate was firstly decreased and then increased after soaking the seeds with CaCl2.The results suggested that soaking seeds with CaCl2 could alleviate the injury of salt stress to some extent.When the concentration of CaCl2 is 20 mmol/L,the remission effect is the best,it significantly re-duced the harm of salt stress on cotton seed germination,seedling growth,antioxidant capacity and cell membrane,cotton seedling had also been become more tolerant to salt stress.
Exogenous CaCl2Salt stressSeed germinationActivity of antioxidant enzymes
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