Preliminary Identification of Candidate Genes withVirscent Gene in Cotton Based on BSA-Seq
Virescent trait could be used as a natural marker for breeding to reduce time and process of hybrid breeding,moreover they were ideal model systems to study chlorophyll and chloroplast biosynthesis and biodegradation process.the virescent mutant in cot-ton is of great theoretical and practical implications.In this study,an F2 segregation population was constructed derived from 115-23(virscent mutant)and JSH1527(Normal green trait),based on the identification of virescent trait,30 virescent and 30 green plants were selected to construct a mixed pool,four samples(2 parent pools and 2 mixed pools)were sequenced,SNP-index were used for association analysis to determine the candidate regions of virescentgenes.As a result,840,612 SNPs were obtained between the two parents,one candidate physical regions showing confidence indices higher than 95%were obtained on chromosome D02,which con-tained 93 annotated genes,four of these genes were functional annotations related to chloroplast synthesis and may be candidate genes for the regulation of cotton leaf color.This study laid a foundation for further analyzing the genetic mechanism and clones of genes of cotton virescent formation.
NETL
NSTL
万方数据
维普
