Study on Hairy Root Induction of of lantago asiatica L.
In order to establish the Ri plasmid mediated lantago asiatica L.transformation system,hairy roots were induced by the explants of Agrobacterium rhizoides A4 and R1601 infected by Agrobacterium rhizoides.The results showed that 8 min was the best infection time for plantago explants.The two bacterial solutions can induce hairy roots in front leaves,petioles and roots,and the hairy roots in front leaves and petioles are less than 40％,and hairy roots in front roots are more than 80％.After molecular identification,rolB gene of Ri plasmid T-DNA was successfully integrated into hairy root,indicating that the root of plantain was the best explants suitable for hairy root induction,which could lay a foundation for future expansion of hairy root culture.
万方数据
维普
