Exploration of the anti-inflammatory mechanism of Danxi gout formula in gouty arthritis based on net-work pharmacology
Objective To explore the anti-inflammatory effect and mechanism of DanxiTongfeng Decoction(DXTFD)in the treatment of gouty arthritis(GA)with network pharmacology,molecular docking technology and animal experiments.Methods The TCMSP database was used to identify active components and targets of DXTFD;TTD,DrugBank,OMIM,and GeneCards databases were used to screen for GA-related targets.After obtaining the intersection of these targets,a protein-protein interaction(PPI)network was constructed using Cytoscape3.8.0 and STRING databases.Core targets were subjected to GO enrichment analysis and KEGG pathway en-richment analysis using R language.Molecular docking was performed with AutodockVina 1.5.7 to predict the binding ability of active components and disease targets.For some of the core targets of network pharmacology screening(IL-6,IL-8),we designed GA rat model animal experiment to detect the pathomorphology and serum inflammatory factors(IL-6,IL-8)of GA rat model to further verify the anti-inflammatory effect and mechanism of Danxi gout prescription in the treatment of gouty arthritis.Results A total of 132 DXT-FD active ingredients and 776 targets were identified for DXTFD,with 375 disease targets and 99 intersection targets.GO enrichment a-nalysis indicated significant involvement in inflammatory response,steroid metabolism,fatty acid metabolism and other biological regula-tion.KEGG enrichment analysis revealed 132 signaling pathways,involved in the NOD-Like receptor signaling pathway.The active in-gredients gerloin and baicalein demonstrated strong binding ability with TNF,IL-6,PTGS2 and MAPK3 targets.Animal experiments in-dicated that DXTFD could exert therapeutic effects by inhibiting the expression of inflammatory cytokines IL-6 and IL-8 in GA rats.Conclusion DXTFD may prevent and treat GA by inhibiting inflammation with multiple components and multiple targets.
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