Cloning and Expression Analysis of GhERF8 Gene in Gossypium hirsutum L.
The ethylene-responsive element-binding factor (ERF) gene family is one of the largest transcription factor gene families in plants. In this study, a novel ERF gene named GhERFS (GenBank: JN656957) was isolated from our laboratory's leaf cD-NA library for Gossypium hirsutum L. The gene encodes a putative of 265 amino-acid protein with a conserved AP2 domain. To understand the function of GhERFS, real-time quantitative PCR (RT-PCR) was used to evaluate the relative expression of GhERFS at different developmental stages of leaves and tissues. The results showed that GhERFS could express in all tissues, but expression levels differed. The expression of GhERFS was the highest in the later stage of leaf maturation. During leaf senescence, GhERF8 expression and chlorophyll content were inversely correlated, indicating that GhERFS could be related to leaf senescence. Under ethephon and jasmonic acid (JA) treatment, the expression of GhERFR was up-regulated in leaves, but there was no significant change in expression of GhERFS under abscisic acid (ABA) treatment. These results indicated that GhERFS could participate in the signal transduction of ethylene and JA, but not in the ABA-dependent pathway.
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