QTL mapping and genetic analysis of cadmium stress tolerance in upland cotton
[Objective]Cotton is a relatively tolerant crop to cadmium(Cd)stress.It is of great significance to breeding and extending the Cd tolerant cotton cultivars for remediation of Cd contaminated soil.This study aims to analysis the inheritance of cotton tolerance to Cd stress and to map the quantitative trait locus(QTL)related to Cd stress.[Methods]In this study,188 recombinant inbred lines(RIL)called HM188 derived from an intraspecific hybrid between HS46 and MARCABUCAG8US-1-88 were used to study the phenotypic data of germination rate,germination potential,chlorophyll content,plant height,dry and fresh weight at seedling stage under Cd stress,and the Cd stress coefficient of each material was calculated as well.QTL mapping was conducted for each trait and Cd stress coefficient of HM188 population by using complete interval mapping method.[Results]According to the performance and Cd stress coefficient of each trait under Cd stress,MARCABUCAG8US-1-88 was the Cd tolerant parent,while HS46 was a sensitive one.The Cd tolerance of 188 RIL was basically normal distribution,including 23 strong Cd tolerant lines,49 Cd tolerant line,67 medium tolerant lines,and 49 sensitive lines.A total of 28 QTL for Cd stress coefficient was identified,which were distributed on 14 chromosomes with explaining of variation of 1.58%～8.41%.Among them,qRFW-13-1 related to root fresh weight was detected in both environments,which explained variation of 7.66%and 7.71%,respectively.[Conclusion]Cd tolerance in upland cotton is a quantitative trait controlled by multiple genes,which was greatly affected by the environment.However,qRFW-13-1 for Cd tolerance may be a stable QTL,which has some application values in molecular breeding for Cd tolerant cotton.
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