[Objective]This research aims to analysis the function of GhWRKY44 gene under drought stress,and to provide candidate gene resources for drought-resistant breeding in cotton.[Methods]The coding sequence of GhWRKY44 gene was obtained by polymerase chain reaction(PCR)from the cDNA of CQJ-5(Gossypium hirsutum)leaves.And bioinformatics analysis was performed.The expression pattern of GhWRKY44 gene under the treatment of abscisic acid(ABA)and polyethylene glycol(PEG)6000 were analyzed by quantitative real-time PCR(qRT-PCR).The function of GhWRKY44 genes under drought stress was investigated by using the virus-induced gene silencing(VIGS)technology.[Results]The protein encoded by GhWRKY44 is a member of class Ⅰa WRKY,and is closely related to GbWRKY44.The expression of GhWRKY44 was induced by PEG 6000 and ABA.Compared with the control cotton plants,GhWRKY44 silenced cotton plants showed more severe leaf wilting,and plant survival rate and leaf chlorophyll content(soil and plant analyzer development,SPAD value)were significantly reduced under drought stress.With 6 h and 7 h of dehydration treatment,the leaf water loss rate of Gh WRKY44 silenced cotton plants was significantly higher than that of control plants.[Conclusion]Silencing of Gh WRKY44 gene reduced drought tolerance of cotton,and Gh WRKY44 is a positive regulator of drought tolerance in cotton.
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