Comprehensive evaluation of two salt tolerance identification methods and salt tolerance identification of glandless cotton germplasms
[Objective]This study aims to explore the response of Gossypium hirsutum under artificial seawater and NaCl stresses,and to assess the feasibility of using artificial seawater to simulate salt stress for evaluating salt tolerance of cotton germplasms.[Methods]Utilizing 135 distinct G.hirsutum germplasms as the experimental materials,this study investigated the impact of artificial seawater and NaCl stresses on cotton seed germination and seedling growth.Through the integration of principal component analysis,membership function analysis,and cluster analysis methodologies,the comprehensive evaluation of cotton salt tolerance was conducted.The results of two identification methods were verified by field experiments under natural salt stress.[Results]The consistency of the identification results under the two salt stresses were only 52.38％,and there were great differences in the results.The identification results under artificial seawater stress were significantly and positively correlated with the results of field experiments,with a correlation coefficient of 0.720;while that under NaCl stress were not significantly correlated with the field identification results.Under artificial seawater and NaCl stress treatments,21.90％and 33.33％of the 105 glandless cotton germplasms were resistant or tolerant to salt stress,respectively.Among them,Lu 17 and Handifen 29 showed strong salt tolerance under the two salt treatments.[Conclusion]Using artificial seawater that simulates the composition of coastal soil can identify the salt tolerance of cotton germplasms more accurately.Glandless cotton generally exhibits poorer salt tolerance,but there are still some germplasms with strong salt tolerance that can be used to breed new salt tolerant glandless cotton cultivars.
万方数据
维普
