Genome-wide association study of cotton lint percentage and boll weight
[Objective]This study aims to perform genome-wide asscciation study of cotton yield traits,such as lint percentage(LP)and boll weight(BW),and to mine the candidate genes,and may be helpful for improving cotton yield through molecular marker-assisted selection and molecular design breeding.[Methods]Genome-wide association study was performed using 300 upland cotton germplasms resequencing(10×)data and 3 055 642 high-quality single nucleotide polymorphism(SNP)for LP and BW in five environments and best linear unbiased predictive value(BLUP)for two years to detect significant association loci and candidate genes.[Results]The cotton LP and BW showed wide variations in different environments,with an average coefficient of variation of 9.40％and heritability of 92.81％for LP,and an average coefficient of variation of 11.96％and heritability of 86.67％for BW.BW is significantly positively correlated in different environments.It's the same with LP.Population structure analysis,principal component analysis,and phylogenetic analysis classified the 300 upland cotton lines into six subgroups.Genome-wide association study detected a total of 223 quantitative trait locus(QTL)associated with LP and 89 QTL associated with BW.The three stable QTL qLP_Gh5.18,qLP_Gh12.43,and qLP_Gh17.2 screened in LP were further analyzed,and 17 related candidate genes were found.Two stable QTL qBW_Gh7.5 and qBW_Gh19.5 related to BW were further analyzed,and 8 related candidate genes were identified.[Conclusion]Five stable QTL were identified in 300 up land cotton lines associated with cotton LP and BW,and a total of 25 candidate genes related to LP and BW were mined.
Gossypium hirsutum L.lint percentageboll weightgenome-wide association study
国家科技期刊平台
NSTL
万方数据
