Simultaneous determination of five plant hormones in cotton leaves using QuEChERS combined with HPLC?MS/MS

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国家科技期刊平台
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外文摘要：Background Plant hormones profoundly influence cotton growth,development,and responses to various stresses.Therefore,there is a pressing need for an efficient assay to quantify these hormones in cotton.In this groundbreak-ing study,we have established QuEChERS-HPLC‒MS/MS method,for the simultaneous detection of multiple plant hormones in cotton leaves,allowing the analysis and quantification of five key plant hormones.Results Sample extraction and purification employed 0.1%acetic acid in methanol and C18 for optimal recov-ery of plant hormones.The method applied to cotton demonstrated excellent linearity across a concentration range of 0.05-1 mg·L-1,with linear regression coefficients exceeding 0.99.The limits of quantification(LOQs)were 20 μg·kg-1 for GA3 and 5 μg·kg-1 for the other four plant hormones.Recovery rates for the five plant hormones matrix spiked at levels of 5,10,100,and 1000 μg·kg-1 were in the range of 79.07%to 98.97%,with intraday relative stand-ard deviations(RSDs)ranging from 2.11%to 8.47%.The method was successfully employed to analyze and quantify the five analytes in cotton leaves treated with plant growth regulators.Conclusion The study demonstrates that the method is well-suited for the determination of five plant hormones in cotton.It exhibits excellent selectivity and sensitivity in detecting field samples,thus serving as a robust tool for in-depth research into cotton physiology.

外文关键词：

CottonPlant hormonesQuEChERSHPLC‒MS/MS

作者：

WANG Wenhao、SONG Xianpeng、WANG Dan、MA Yajie、SHAN Yongpan、REN Xiangliang、HU Hongyan、WU Changcai、YANG Jun、MA Yan

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作者单位：

National Key Laboratory of Cotton Biobreeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China

College of Agriculture,Hebei Agricultural University,Baoding,Hebei 071000,China

National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China

Zhengzhou Research Base,National Key Laboratory of Cotton Biobreeding and Integrated Utilization,Zhengzhou University,Zhengzhou,Henan 450001,China

Western Agricultural Research Center,Chinese Academy of Agricultural Sciences,Changji,Xinjiang 831100,China

College of Agriculture,Hebei Agricultural University,Baoding Hebei 071000,China

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基金：

National Key R&D Program of ChinaAgricultural Science and Technology Innovation Program of Chinese Academy of Agricultural SciencesChina Agriculture Research System

项目编号：

2022YFD1400300

出版年：

2024

DOI：

10.1186/s42397-024-00179-w

棉花研究(英文)

中国农业科学院棉花研究所,中国农学会

棉花研究(英文)

ISSN：2096-5044

年,卷(期)：2024.7(2)