首页|Morphological characteristics,developmental dynamics,and gene temporal expressions across various development stages of Aphis gossypii sexual female

Morphological characteristics,developmental dynamics,and gene temporal expressions across various development stages of Aphis gossypii sexual female

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Background Aphis gossypii(Hemiptera:Aphididae)is a worldwide polyphagous phloem-feeding agricultural pest,and it can produce offspring by sexual or asexual reproduction.Compared with dozens of generations by partheno-genesis,sexual reproduction is performed in only one generation within one year,and little is known about the sexual reproduction of A.gossypii.In this study,sexual females of A.gossypii were successfully obtained through a previ-ously established induction platform,and the morphological characteristics,developmental dynamics,and temporal gene expression were examined.Subsequently,signaling pathways potentially involved in regulating the growth,development,and reproduction of sexual females were investigated.Results The morphological observation showed that from the 1st instar nymph to adult,sexual females exhibited a gradually deepened body color,an enlarged body size,longer antennae with a blackened end,and obviously pro-truding cauda(in adulthood).The anatomy found that the ovaries of sexual females developed rapidly from the 2nd instar nymph,and the embedded oocytes matured in adulthood.In addition,time-course transcriptome analysis revealed that gene expression profiles across the development of sexual females fell into 9 clusters with distinct pat-terns,in which gene expression levels in clusters 1,5,and 8 peaked at the 2nd instar nymphal stage with the largest number of up-regulated genes,suggesting that the 2nd instar nymph was an important ovary development period.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that a large number of genes in the sexual female adult were enriched in theTGF-beta signaling pathway and Forkhead box O(FoxO)signaling pathway,highlighting their important role in sexual female adult development and reproduction.Conclusion The morphological changes of the sexual female at each developmental stage were revealed for the first time.In addition,time-course transcriptomic analyses suggest genes enriched in the TGF-beta signaling pathway and FoxO signaling pathway probably contribute to regulating the development and oocyte maturation of sexual females.Overall,these findings will facilitate the regulating mechanism research in the growth and development of sexual females by providing candidate genes.

Aphis gossypiiSexual femaleGrowth and developmentOogenesisTime-course transcriptome

LÜ Jingli、WANG Liuyu、ZHANG Kaixin、LI Dongyang、GAO Mengxue、GUO Lixiang、TANG Zhijuan、GAO Xueke、ZHU Xiangzhen、WANG Li、JI Jichao、LUO Junyu、CUI Jinjie

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Research Base of Zhengzhou University,State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China

State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization,School of Agri-cultural Sciences,Zhengzhou University,Zhengzhou,Henan 450001,China

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China

Western Agricultural Research Center,Chinese Academy of Agricultural Sciences,Changji,Xinjiang 831100,China

State Key Laboratory of Plant Stress Biology,College of Life Sciences,Henan University,Kaifeng,Henan 450046,China

College of Horticulture and Landscape Architecture,Tianjin Agricultural University,Tianjin 300392,China

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2024

10.1186/s42397-024-00201-1

棉花研究(英文)
中国农业科学院棉花研究所,中国农学会

棉花研究(英文)

ISSN：2096-5044
年,卷(期)：2024.7(4)