CTA中空纤维亲和色谱的制备及其吸附性能
Preparation and adsorption properties of CTA hollow fiber affinity chromatography
李淑琴 1武皎洁 1周君婕 1魏永明 1许振良 1杨虎1
作者信息
- 1. 化学工程联合国家重点实验室,华东理工大学,功能膜科学与工程研究中心,上海 200237
- 折叠
摘要
为探究三醋酸纤维素(CTA)中空纤维亲和色谱的吸附性能,以CTA中空纤维膜为基膜,通过水解、1,4-丁二醇二缩水甘油醚(EGDE)交联活化、接枝1,6-己二胺(HMDA)作为间隔臂对膜表面进行改性,然后以亚氨基二乙酸(IDA)为配基,螯合Cu2+制备了改性CTA中空纤维亲和色谱.对于γ-球蛋白的静态吸附研究表明:当吸附液γ-球蛋白质量浓度为1 mg/mL、离子浓度为0.2 mol/L和pH=8时,有最大静态吸附容量2.4 mg/cm3.且其吸附过程符合Langmuir等温吸附模型.对于γ-球蛋白的动态吸附研究表明:在实验研究的范围内,不同流速下的穿透曲线都呈现出类似"S"型,流速并不影响CTA中空纤维亲和色谱上γ-球蛋白的结合能力;γ-球蛋白的动态吸附容量随其初始浓度的增加而增加.
Abstract
In order to explore the adsorption properties of cellulose triacetate(CTA)hollow fiber affinity chromatography,the CTA hollow fiber membrane was used as the basal membrane,The surface of the membrane was modified by hydrolysis,crosslinking activation of 1,4-butanediol diglycidyl ether(EGDE),grafting 1,6-hexanediamine(HMDA)as the spacer arm,and then the modified CTA hollow fiber affinity chromatography was prepared by chelating Cu2+with iminodiacetic acid(IDA)as ligand.In the static adsorption study of γ-globulin:when the concentration of γ-globulin adsorption solution is 1 mg/mL,ion concentration is 0.2 mol/L and pH=8,the maximum static adsorption capacity is 2.4mg/cm3.The adsorption process accords with Langmuir isothermal adsorption model.For the study of dynamic adsorption of γ-globulins:within the scope of experimental studies,the penetration curves at different flow rates were similar to"S"shape,and the flow rate did not affect the binding ability of γ-globulin on CTA hollow fiber affinity chromatography;The dynamic adsorption capacity of γ-globulin increased with the increase of initial concentration.
关键词
表面改性/CTA中空纤维亲和色谱/静态吸附/动态吸附Key words
surface modification/CTA hollow fiber affinity chromatography/static adsorption/dynamic adsorption引用本文复制引用
基金项目
国家自然科学基金(21978082)
国家自然科学基金(22278132)
国家自然科学基金(22078092)
出版年
2024
NETL
NSTL
维普
万方数据