Preparation and adsorption properties of CTA hollow fiber affinity chromatography
In order to explore the adsorption properties of cellulose triacetate(CTA)hollow fiber affinity chromatography,the CTA hollow fiber membrane was used as the basal membrane,The surface of the membrane was modified by hydrolysis,crosslinking activation of 1,4-butanediol diglycidyl ether(EGDE),grafting 1,6-hexanediamine(HMDA)as the spacer arm,and then the modified CTA hollow fiber affinity chromatography was prepared by chelating Cu2+with iminodiacetic acid(IDA)as ligand.In the static adsorption study of γ-globulin:when the concentration of γ-globulin adsorption solution is 1 mg/mL,ion concentration is 0.2 mol/L and pH=8,the maximum static adsorption capacity is 2.4mg/cm3.The adsorption process accords with Langmuir isothermal adsorption model.For the study of dynamic adsorption of γ-globulins:within the scope of experimental studies,the penetration curves at different flow rates were similar to"S"shape,and the flow rate did not affect the binding ability of γ-globulin on CTA hollow fiber affinity chromatography;The dynamic adsorption capacity of γ-globulin increased with the increase of initial concentration.
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