Study on cell retention technologies of perfusion culture based on hollow fiber
Recently,due to the increasing demand for protein products in the bio-pharmaceutical industry,the perfusion culture mode of continuous cell culture technology has been increasingly valued.To maintain a high number of cells and productivity in bioreactors and harvest high-purity protein products,a good separation device is needed to retain the cells in the bioreactor.Based on comparing different types of cell retention technologies,this article summarizes the advantages of hollow fiber filtration technologies,such as mild operating conditions,high flux,high filtration accuracy,and wide application.In order to alleviate the membrane fouling problem in the process of hollow fiber filtration and extend the lifespan of membrane modules,this article also analyzes the design factors such as hollow fiber material,pore size,module inner diameter,length,and packing density commonly used in perfusion culture,and discusses the influence of different tangential flow filtration operation parameters on membrane modules.To provide a theoretical basis for the design and optimization of hollow fiber membrane modules for cell separation in the field of perfusion culture technology.
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