Effect of type 2 myeloid cell trigger receptor on glial cell inflammation based on the phosphoinositide-3-kinase/protein kinase B/mammalian target of sirolimus pathway
Effect of type 2 myeloid cell trigger receptor on glial cell inflammation based on the phosphoinositide-3-kinase/protein kinase B/mammalian target of sirolimus pathway
李莉 1秦迎辉 1于广娜 1陈庆友 1黄丽娟 1齐婷婷 1朱丽娟1
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作者信息
1. 齐齐哈尔医学院附属第三医院神经内科,黑龙江 161000
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摘要
目的 探究基于磷脂酰肌醇-3-激酶(phosphoinositide-3-kinase,PI3K)/蛋白激酶B(protein kinase B,AKT)/哺乳动物西罗莫司靶蛋白(mammalian target of sirolimus,mTOR)通路途径髓样细胞触发受体2(trig-gering receptors expressed on myeloid cells-2,TREM2)对胶质细胞炎症的影响.方法 对胶质细胞采用脂多糖(lipopolysaccharide,LPS)进行诱导造模后完成分组检测,分组如下:对照组、LPS处理组和TREM2组,通过RT-PCR方法检测白细胞介素(interleukin)-6、IL-1β和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)中mRNA表达水平,采用免疫印迹法检测各组细胞PI3K、AKT、mTOR蛋白表达水平.结果 与对照组比较,LPS处理组、TREM2组IL-6、IL-1β和TNF-α中mRNA表达水平均升高,TREM2组 IL-6、IL-1β、TNF-α中mRNA表达水平高于LPS处理组,差异有统计学意义(P<0.05).LPS处理组和TREM2组细胞PI3K、AKT和mTOR蛋白表达水平均高于对照组,TREM2组细胞PI3K、AKT和mTOR蛋白表达水平高于LPS处理组,差异有统计学意义(P<0.05).结论 小胶质细胞过度活化后诱导神经炎症,P13K/AKT/mTOR信号通路参与多种神经退行性疾病的发生发展过程,其在细胞炎症反应中都发挥着重要作用.
Abstract
Objective To explore the effect of triggering receptors expressed on myeloid cells-2(TREM2)on inflammatory cells based on the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)/mam-malian target of rapamycin(mTOR)pathway.Methods Glial cell cultures were induced with lipopolysac-charide(LPS),then divided into the following groups:control group;LPS-treated group;and TREM2 group.The mRNA expression levels of interleukin-6(IL-6),IL-1β,and tumor necrosis factor-alpha(TNF-α)were detected by reverse transcription-polymerase chain reaction(RT-PCR).Western blot analy-sis was used to detect the protein expression levels of PI3K,AKT,mTOR in each group.Results The mRNA expression levels of IL-6,IL-1β and TNF-α were increased in the LPS-treated group and the TREM2 group compared to the control group,the mRNA expression levels of IL-6,IL-1β and TNF-α in the TREM2 group were higher than those in the LPS-treated group(all P<0.05).Western blot analysis showed that the protein expression levels of PI3K,AKT and mTOR in the LPS-treated group and the TREM2 group were higher than those in the control group,the protein expression levels of PI3K,AKT and mTOR in the TREM2 group were significantly increased compared to the LPS-treated group(all P<0.05).Conclusions Neuroinflammation induced by excessive activation of microglia,the P13K/AKT/mTOR signaling pathway is involved in the occurrence and development of several neurodegenerative diseases,which plays an important role in the cellular inflammatory response.
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