三阴性乳腺癌患者长链非编码核糖核酸膀胱癌相关转录本1和miR-519d及三结构域蛋白32的表达及相关性

Expression and correlation analysis of long non-coding RNA bladder cancer-associated transcript 1,miR-519d,and tripartite motif protein 32 in patients with triple-negative breast cancer

邢文婷 ¹陈小华 ²何秉征 ¹蔺小艳 ¹杨璐 ³赵翰林 ³陈诚⁴

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作者信息

1. 兰州大学第一医院康复医学科,甘肃 730030
2. 兰州大学第一医院放疗科,甘肃 730030
3. 兰州大学第一临床医学院,甘肃 730030
4. 兰州大学第一医院乳腺病科,甘肃 730030
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摘要

目的探讨三阴性乳腺癌患者血清中长链非编码核糖核酸膀胱癌相关转录本 1(long non-coding RNA bladder cancer-associated transcript 1,lncRNA-BLACAT1)、miR-519d、三结构域蛋白 32(tripartite motif protein 32,TRIM32)的表达及相关性.方法于2021年6月至2022年7月在兰州大学第一医院就诊的罹患乳腺癌且具有完整随访资料的患者中,选取经病理检查和免疫组化证实为三阴性乳腺癌且癌旁5cm以外为正常组织的52例患者纳入观察组,选择同期体检的50例健康受试者纳入对照组.反转录聚合酶链反应(reverse-transcription polymerase chain reaction,RT-PCR)和免疫印迹法检测两组研究对象外周血lncRNA-BLACAT1、miR-519d和TRIM32的mRNA和蛋白表达水平,分析lncRNA-BLACAT1与miR-519d、TRIM32的相关性.结果三阴性乳腺癌组lncRNA-BLACAT1、TRIM32的mRNA表达水平较对照组明显升高,miR-519d的mRNA表达水平较对照组明显下降,差异有统计学意义(P<0.05).三阴性乳腺癌组TRIM32蛋白表达水平较对照组明显升高,差异有统计学意义(P<0.05).Pearson相关分析结果显示,三阴性乳腺癌患者血清中lncRNA-BLACAT1与miR-519d的mRNA表达水平成负相关(r=-0.850,P<0.05),血清中lncRNA-BLACAT1与TRIM32的mRNA表达水平成正相关(r=0.842,P<0.05).结论三阴性乳腺癌患者血清中lncRNA-BLACAT1与TRIM32水平升高,miR-519d水平下降,lncRNA-BLACAT1、TRIM32与miR-519d可作为诊断三阴性乳腺癌的潜在分子标志物.

Abstract

Objective To determine the expression and correlation of long non-coding RNA bladder cancer-associated transcript 1(lncRNA-BLACAT1),miR-519d,and tripartite motif protein 32(TRIM32)in patients with triple-negative breast cancer.Methods Among the patients with breast cancer who received treatment in the First Hospital of Lanzhou University from June 2021 to July 2022 and had complete fol-low-up data,52 patients who were diagnosed with triple-negative breast cancer and had normal distant tis-sue≥5 cm from the edge of the cancer confirmed by pathologic examination and immunohistochemistry were included in the observation group.Fifty healthy subjects who underwent physical examinations during the same period were included in the control group.The expression of lncRNA-BLACAT1,miR-519d,and TRIM32 mRNA and protein in the peripheral blood of patients in the two groups was detected by reverse-transcription polymerase chain reaction(RT-PCR)and immunoblotting.Results Compared with the con-trol group,the level of lncRNA-BLACAT1 and TRIM32 mRNA expression in patients with triple-negative breast cancer was significantly increased.In patients with triple-negative breast cancer,the level of miR-519d expression was significantly decreased.These comparisons were all statistically significant(P<0.05).The level of TRIM32 protein expression in triple negative breast cancer group was significantly higher than the control group,and the difference was statistically significant(P<0.05).Pearson correlation analysis showed that the level of lncRNA-BLACAT1 expression in the serum of patients with triple-negative breast cancer was negatively correlated with the level of miR-519d expression(r=-0.850,P<0.05),the level of lncRNA-BLACAT1 expression was positively correlated with the level of TRIM32 expression(r=0.842,P<0.05).Conclusions In patients with triple-negative breast cancer,the level of lncRNA-BLACAT1 and TRIM32 expression is increased,while the level of miR-519d expression is significantly decreased.LncRNA-BLACAT1,miR-519d,and TRIM32 might serve as potential molecular markers in the diagnosis of triple-negative breast cancer.

关键词

三阴性乳腺癌/长链非编码核糖核酸膀胱癌相关转录本1/miR-519d/三结构域蛋白32

Key words

Triple negative breast neoplasms/Long non-coding RNA bladder cancer-associated transcript 1/miR-519d/Tripartite motif protein 32 protein

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基金项目

兰州大学学生创新创业行动计划项目(20230060065)

出版年

2023

慢性病学杂志

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参考文献量19

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