The effects of fructo-oligosaccharide on the intestinal mucosa of the mouse with inflammatory bowel disease
To explore effect of fructo-oligosaccharide (FOS) on the intestinal mucosa of mice,30 mice were divided into control group,DSS group,and FOS group.The mice in groups DSS and FOS were fed with 5％ DSS to induce inflammatory bowel disease and then the mice in group FOS were fed with 5％ FOS.Disease activity index (DAI) of mice was calculated;the expression of IL-6,IL-33,TNF-α and Reg Ⅲ γ in colon of each group was measured at the end of experiment.The changes of colon length and histological damage score and bacteria were measured too.The mice in group FOS showed a lower DAI (P＜0.000 1) and histological damage score (P＜0.000 1)as compared with group DSS.The colon texture of mice were hardened and the wrinkles of colon were disappeared (P＜0.01) in group DSS.The mice in group FOS showed a less pathogenic bacteria as compared with group DSS(P＜ 0.01).The levels of IL-6 (P＜0.05),IL-33 (P＜0.05),TNF-α and Reg Ⅲ γin colon of group FOS were lower than those in DSS at the end of experiment,while the difference of TNF-α and Reg Ⅲ γlevels between the two group demonstrated no statistical significance.Taken together,treatment with FOS can improve the ratio of beneficial bacteria and reduce the expression of IL-6 and IL-33,thus decrease colonic inflammatory response in DSS induced colitis.
NETL
NSTL
万方数据
维普
