F.nucleatum promotes colorectal cancer metastases via macrophage STAT6 O-GlcNAcylation
This study was designed to explore the role of F.nucleatum in macrophage polarization and colorectal cancer metastasis.F.nucleatum and RAW264.7 cells were cocultured at the MOI of 100∶1,and then glucose uptake was measured by 2-NBDG,expression levels of M1 and M2 macrophage markers were determined by qPCR,glycosylation modification level and polarization marker expression of macrophages were tested by Western blotting,and STAT6 glycosylation modification was verified by Co-IP experiment.Meanwhile,F.nucleatum was co-cultured with RAW264.7(MOI=100∶1),and the supernatant was removed to treat RKO cells.The migration ability of colorectal cancer was detected by transwell experiment and scratch experiment.Data showed that F.nucleatum promoted M2 macrophage polarization and enhance the metastasis of colorectal cancer cells RKO;F.nucleatum also promoted the glucose intake of macrophages and enhanced the expression of M2-related markers.Taken together,F.nucleatum promotes the level of glycosylation in macrophages,which induces its polarization to M2 by modifying O-GlcNAc of STAT6,to regulate colorectal cancer metastasis.
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