Roles of osteopontin in the immune regulation of multilocular echinococcosis infection by regulating LPS/TLR4 signaling pathway
The purpose of this study is to investigate the role of osteopontin in modulating the LPS/TLR4 signaling pathway during the immune response to multilocular echinococcosis infection.A total of forty female Balb/c mice were recruited and divided into four distinct groups:EmOPN silencing group,EmOPN overexpressing group,model group,and control group.Multilocular echinococcosis model was established in mice,and the mice were treated accordingly.The EmOPN silencing group received lentivirus LV-EmOPN-734,the EmOPN overexpressing group received lentivirus LV-EmOPN-0423,the model group was injected with normal saline,while the control group did not receive any modeling treatment and was also injected with normal saline.After two weeks of infection,various parameters were measured,including liver tissue sections,spleen coefficients,and splenic lymphocyte counts.Additionally,apoptosis in splenic cells was analyzed using flow cytometry,and the expression levels of LPS and TLR4 signaling pathway proteins in liver tissues were assessed by qRT-PCR and Western blot techniques.Data showed that there are significant variations among the four groups in terms of spleen index,splenic lymphocyte count,and apoptotic rates.Notably,the EmOPN overexpressing group exhibited significantly higher spleen index and lymphocyte count compared to the other groups.Liver tissues from infection mice of the three groups displayed irregular white lesions with infiltrative growth,whereas the liver tissue of the control group appeared normal.Moreover,the EmOPN overexpressing group displayed remarkable differences in the mRNA levels of cytokines like IL-1β and IFN-γ,as well as immune response-related genes such as CCR7,MRC1,and Retnla.Additionally,significant differences in protein expression levels of LPS and TLR4 were observed in the four groups.Taken together,in alveolar echinococcosis,liver and spleen functions are impaired.And osteopontin has the potential to enhance the LPS/TLR4 signaling pathway,thereby correcting macrophage immune dysfunction.
OsteopontinLipopolysaccharideToll-like receptor 4Alveolar echinococcosisImmune function
万方数据
维普
