Immunoregulatory effect of STING pathway on regulatory T cells in glioma mice
Objective To explore the regulatory effect of STING pathway on the immunity related to regulatory T cells(Treg)in glioma mice.Methods Glioma models were constructed based on ectopic GL261 implantation of cell lines established from ectopic brain implantation in mice.After successful modeling,the mice were randomly divided into control group,SN-011 group and MSA-2 group.STING was inhibited by intracranial injection of SN-011(10 mg/ml)in group SN-011,and promoted by intracranial injection of MSA-2(50 mg/ml)in group MSA-2.The effects of STING on tumor volume,mass and immune factors IFN-β and IL-10 were compared.The infiltrated Treg cells in tumor tissues were observed by immunohistochemical staining with FOXP3,and the expression of STING and IRF3 proteins were detected.Results Compared with the control group,the tumor volume,tumor mass,IL-10 level and Treg invasion ratioin SN-011 group were increased(P<0.05),while the protein levels of IFN-β,STING and IRF3 were decreased(P<0.05);Compared with the control group,the tumor volume,tumor mass,IL-10 level and Treg invasion ratio in the MSA-2 group were decreased(P<0.05),while IFN-β,STING and IRF3 protein levels were increased(P<0.05).Compared with SN-011 group,tumor volume,mass,IL-10 level and Treg invasion ratio in MSA-2 group were decreased(P<0.05),while IFN-β,STING and IRF3 protein levels were increased(P<0.05).Conclusion STING pathway promotes the progression of brain glioma by promoting Treg infiltration and inducing immune escape.
Lung cancerInterleukin-6ImmunityRegulatory T cells
万方数据
维普
