Regulation of TLR4/NF-κB pathway in tumor-associated fibroblasts by lipopolysaccharide promotes EMT in colon cancer cells
Objective To investigate the effect of TLR4/NF-κB pathway on epithelial mesenchymal transformation of colon cancer cells in tumor-associated fibroblasts.Methods A co-culture model of colon cancer cells and CAFs cells was established.Colon cancer cells were taken as control group,co-culture model and lipopoly Saccharide(LPS,15 μg/ml)co-culture model were taken as experimental group,and the migration of colon cancer cells was observed by transwell assay.the expressions of α-SMA,FAP and VEGF in CAFs cells were detected by immunofluorescence.the protein and mRNA expressions of TLR4、Myd88、NF-κB in CAFs cells and E-cadherin,N-cadherin、Vimentin in colon cancer cells were detected by Western blotting and RTPCR,respectively.Results The co-culture model of CAFs cells and colon cancer cells can promote the migration of colon cancer cells,and increase the expression of α-SMA and FAP in CAFs cells.the migration of colon cancer cells is related to the activation of TLR4/NF-κB pathway in CAFs cells,which enhances the expression of VEGF in CAFs cells,further promotes EMT of colon cancer cells,and increases the migration and invasion ability of cancer cells.Conclusion CAFs can promote the migration of colon cancer cells,and the mechanism may be related to the activation of TLR4/NF-κB pathway.CAFs express VEGF cytokines,thereby promoting epithelial mesenchymal transformation of colon cancer cells.
Tumor-associated fibroblastsColon cancer cellTLR4/NF-κB pathwayEpithelial mesenchymal transformation
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