Protection of y-39983 preconditioning from retinal ischemia-reperfusion injury in rats
AIM: To investigate the neuroprotective effect of rho-associated coiled coil - forming protein kinase (ROCK) inhibitor γ - 39983 on retinal ischemia reperfusion (IR ) injury in rats.METHODS: Sixty adult SD rats were randomly assigned into normal group(n=15), IR group(n=15), saline group ( n= 15), γ-39983 group( n= 15), and the rats in later three groups were induced retinal IR by intra-anterior chamber infusion of saline solution. Rats in the saline and y-39983 groups were intravitreously injected with normal saline and y-39983 respectively, 5 minutes prior to the induction of ischemia. The protein expression of intercellular cell adhesion molecules - 1 (ICAM - 1 ) was determined by immunohistochemical staining. Retrograde labeling of retinal ganalion cells ( RGCs ) with fluorogold was performed to assess the number of RGCs. Histologic studies and electroretinogram were carried out to evaluate retinal damage.RESULTS: The preadminisration of γ-39983 decreased ICAM-1 expression, elevated the survival rate of RGCs and the recoverage rate of amplitude of b wave and O2 wave significantly, and attenuated the IR - induced the thinning of inner retina.CONCLUSION: γ-39983 can protect the rat retina from IR injury at least partially by inhibiting ICAM-1 abnormal expression, suggesting that γ-39983 may have therapeutic potential for the retinal diseases associated with IR.
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