Study on specific imaging of choroidal melanoma cells by novel functionalized upconversion nanomaterials
·AIM:To prepare a novel functionalized upconversion nanomaterial UCNPs-PEG-Tf through an improved method and observe its specific imaging phenomenon to choroidal melanoma cells(OCM-1).·METHODS;NaYF4:Yb/Er(Up-conversion nanoparticles,UCNPs)was Prepared and plasma was treated to carboxylate the surface;then amino polyethylene glycol and transferrin(Tf)were respectively loaded to prepare UCNPs-PEG-Tf.Characterized them accordingly,its biocompatibility was tested accordingly,and the specific fluorescence phenomenon of OCM-1 was detected by fluorescence spectrophotometer and inverted fluorescence microscopy.·RESULTS:Characterization confirmed successful synthesis of UCNPs,UCNPs-PEG,and further loading of Tf to form UCNPs-PEG-Tf.UCNPs-PEG-Tf showed excellent biocompatibility and emitted significant green fluorescence.Under the same conditions,fluorescence intensity measurement and observations from the inverted fluorescence microscope both demonstrated its significant specificity in imaging to OCM-1 cells.·CONCLUSION;The synthesized novel functionalized UCNPs-PEG-Tf nanocomposite showed good biocompatibility and achieve specific imaging to OCM-1 cells.
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