The effects of temporary rearing with edetate disodium on physicochemical indices and bacterial communities of shrimp
Litopenaeus vannamei(L.vannamei)is rich in moisture,protein,enzyme activity and astaxanthin.This shrimp,however,has such a feature in that,after death its head turns black rapidly under the action of polyphenol oxidase(PPO),and then its meat gets rot and deteriorate under the action of microorganisms,thus reducing the qualities for commodity sales quickly.At present,researches on the above problems were focused on adding antioxidants and preservatives to keep the dead shrimps fresh through collaborative temperature control,which has the bottleneck of low consumer acceptance.To this end,in this study live L.vannamei was selected as the research object,and it was temporarily reared with EDTA-2Na.The effects of temporary rearing with EDTA-2Na on basic physical and chemical indices of L.vannamei were explored by analyzing the PPO,total viable count,as well as the color of the shrimps.On this basis,the differences in the bacterial community during temporary rearing of L.vannamei were further explored through the microbiome.The specific research results are as follows:the addition amount of EDTA-2Na and the duration of temporary rearing were inversely proportional to PPO activity and total viable count,and the end point of temporary rearing had a better inhibitory effect.L*(lightness)value of L.vannamei head and abdomen was slightly increased,while a*(green to red)and b*(blue to yellow)values were decreased.The blackness and redness of L.vannamei were effectively inhibited.Besides,EDTA-2Na also exhibited effects on the number and structure of bacteria of L.vannamei during temporary rearing.The abundance and diversity of species were higher when the shrimps were temporary reared in an environment in which EDTA was added at 0.1%or 0.3%and the duration of rearing was 24 hours,which were more conducive to the dynamic balance of flora and better quality.The phylum of L.vannamei hepatopancreas mainly consisted of Bacteroidota,Proteobacteria,Firmicutes,Actinobacteriota,Desulfobacteriota.Proteobacteria was inhibited in the 0.3%-24 h group,indicating that reproduction of a large number of pathogens was inhibited.The main pathway affecting the L.vannamei hepatopancreas was the metabolism.
NETL
NSTL
万方数据
