Anticancer activity of Penicillium exopolysaccharide on CT26 cells from Cyclocarya paliurus endophyte
To better exploit and utilize microbial resources and develop natural products,a fungus with producing exopolysaccharides was screened from the endophytes of Cyclocarya paliurus,identified as Penicillium citrinum,and the Penicillium exopolysaccharides were extracted by water extraction and alcohol precipitation method,and the anticancer activity of the extracellular polysaccharides was studied.The anticancer activity of Penicillium exopolysaccharides was comprehensively evaluated by measuring the effects of Penicillium exopolysaccharides on CT26 activity,ROS oxygen radical released,cell cycle and apoptosis.It was found that Penicillium exopolysaccharides promoted the release of twice the amount of ROS oxygen radical from cells compared with normal cells at high concentrations(800 μg·mL-1),and it caused nucleus shrinkage and chromatin deterioration to induce apoptosis.
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