Simultaneous determination of plant growth regulators residues in citrus and unhusked rice by QuEChERS-UHPLC-MS/MS
A UHPLC-MS/MS analysis method was established for simultaneous determination of 8 plant growth regulators(PGRs)residues in citrus and unhusked rice.The QuEChERS technique was used for extracting and purifying the target sub-stances from the samples,with MRM monitoring mode,and the quantification was carried out using a matrix-matched working curve external standard method.Under the optimized conditions,good linearity was achieved for the 8 PGRs in the linear range,including Chlormequat chloride,Uniconazole,Forchlorfenuron,Ethychlozate,Diethyl aminoethyl hexanoate,Indole-3-butyric Acid,Thidiazuron,2,4-D,the linear correlation coefficients are all more than 0.995,with recovery ranging from 70.1％to 105.6％with the RSD below 10.8％(n=6).The sample matrix type and sensitivity are better than or equivalent to the existing national testing standards,and this method has been successfully applied to the detection of plant growth regulator residues in citrus fruits and unhusked rice samples.The above results show that the method is accurate and sensitive,and can be used for qualitative and quantitative analysis of plant growth regulators residues in citrus fruits and unhusked rice.
plant growth regulatorsUHPLC-MS/MSQuEChERScitrus and unhusked rice
万方数据
维普
