首页|基于SiO2纳米颗粒的无标记化学发光氯霉素适体传感器

基于SiO2纳米颗粒的无标记化学发光氯霉素适体传感器

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经由食物链长期摄入氯霉素残留物会给人体带来各种危害和疾病.因此,建立快速、准确且高灵敏的食品中氯霉素检测方法具有重要意义.采用溶胶-凝胶法制备了表面氨基化的SiO2纳米颗粒,并用X射线衍射、透射电镜和红外光谱对其进行了表征.然后,利用酰胺键和碱基互补配对作用在SiO2纳米颗粒表面依次组装互补DNA链和氯霉素适配体,成功构建了无标记化学发光氯霉素适配体传感器.该传感器制备简便,其中的氯霉素适配体既可作为生物识别元件,又可与苯甲酰甲醛反应产生化学发光信号,无需标记信号分子.该传感器的氯霉素检出限为3.26×10-9 mol·L-1,且选择性较好.将该传感器应用于实际样品中进行加标,回收率在94.67％～103.00％之间,表明该适体传感器可用于食品中氯霉素的检测.
Label-free chemiluminescent chloramphenicol aptamer sensor based on SiO2 nanoparticle
Long-term ingestion of chloramphenicol residue through the food chain is hazardous to human health and can cause diseases.Hence,it is of paramount importance to develop a rapid,precise and highly sensitive method for the detection of chloramphenicol in food.In this study,amino modified SiO2 nanoparticles were prepared using the sol-gel method,and analyzed through X-ray diffraction,transmission electron microscopy and infrared spectroscopy.Then,the label-free chemiluminescent chloramphenicol aptamer sensor was successfully constructed by assembling complementary DNA chains and chloramphenicol aptamers on the surface of SiO2 nanoparticles via amide bond and complementary base pairing,respectively.It was worth men-tioning that the sensor was prepared via a facile process without labeling signal molecules,because that the chloramphenicol aptamers could react with benzoyl formaldehyde to produce chemiluminescence signal while being biorecognition elements.The as-prepared sensor showed a low detection limit of chloramphenicol(3.26 × 10-9 mol·L-1)and good specificity.Further,the proposed sensor was applied to realize samples with recoveries ranging from 94.67％to 103.00％,demonstrating its feasibility of detecting chloramphenicol in food.

Label-freeaptamer sensorchemiluminescenceSiO2 nanoparticlechloramphenicol

严喜鸾、程诗云、肖义陂、陈馨、艾凡荣、郭亮

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南昌大学化学化工学院,南昌 330031

南昌大学药学院,南昌 330031

南昌市洪都中医院,南昌 330006

南昌大学先进制造学院,南昌 330031

南昌大学中德联合研究院,南昌 330031

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无标记 适体传感器 化学发光法 SiO2纳米颗粒 氯霉素

国家自然科学基金资助项目国家自然科学基金资助项目江西省自然科学基金资助项目江西省自然科学基金资助项目江西省杰出青年科学项目江西省重点研究开发计划

823608573196020720212BAB20609320232BAB20508720224ACB21400520232BBE50017

2024

南昌大学学报(理科版)
南昌大学

南昌大学学报(理科版)

CSTPCD
影响因子：0.418
ISSN：1006-0464
年,卷(期)：2024.48(2)
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